Central to Work Package #2 is: 1) To provide a comprehensive assay of all satiety relevant hormone and metabolite signals and their temporal evolution 2) To address how the dynamics of these signals and their interactions are predictive of next-meal energy consumption.

Methodology: Blood samples will be collected at regular intervals throughout the experiment from preload to ad libitum meal. These will be taken according to standard procedures and assayed for satiety hormones and metabolite composition using an explorative approach. We will explicitly model the dynamics of the hormonal cascades, computing temporal derivatives, as well as fitting basis functions to the time series.